Afforestation of semi-arid shrubland reduces biogenic NO emission from soil

Nitric oxide (NO) plays a central role in the formation of tropospheric ozone, hydroxyl radicals, as well as nitrous and nitric acids. There are, however, large uncertainties around estimates of global NO emissions due to the paucity of data. In particular, there is little information on the rate of NO emission and its sensitivity to processes such as land use changes in dry environments. Here we report on a two-year study on the influence of afforestation on soil NO fluxes in the semi-arid afforestation system in Southern Israel (Yatir forest, mean annual precipitation ∼280 mm). Laboratory incubations were carried out under seasonally defined conditions of soil moisture and temperature using soils sampled in different seasons from the native shrubland (taken both under shrub canopy and in the inter-shrub areas), and from the adjacent ∼2800 ha, 40-year-old pine afforestation site. Combining laboratory results with field measurements of soil moisture and temperature, we up-scaled soil-atmosphere NO fluxes to the ecosystem level. The different microsites differed in their annual mean NO release rates (0.04, 0.14 and 0.03 mg m⁻² d⁻¹ for the shrubland under and between shrubs and for the forest, respectively), and exhibited high inter-seasonal variability in NO emission rates (ranging from zero up to 0.25 mg m⁻² d⁻¹ in the wet and dry-rewetting seasons, respectively), as well as in temperature responses. Up-scaling results to annual and ecosystem scales indicated that afforestation of the semi-arid shrubland could reduce soil NO emission by up to 65%.     

High‐level of resistance to spinosad,emamectin benzoate and carbosulfan in populations of Thrips tabaci collected in Israel

BACKGROUND: The onion thrips, Thrips tabaci Lindeman, is a major pest of several crop plants in the genus Allium, such as onions, garlic and chives. In Israel, these crops are grown in open fields and in protected housing. This thrips is usually controlled by the application of chemical insecticides. In recent years, spinosad, emamectin benzoate and carbosulfan have been the major insecticides used for the control of the onion thrips. In the last 4 years, growers of chives and green onion from several regions of Israel have reported a significant decrease in the efficacy of insecticides used to control the onion thrips. RESULTS: The susceptibility of 14 populations of the onion thrips, collected mainly from chives between the years 2007 and 2011, to spinosad, emamectin benzoate and carbosulfan was tested using a laboratory bioassay. The majority of the populations showed significant levels of resistance to at least one of the insecticides. LC₅₀ values calculated for two of the studied populations showed that the resistance factor for spinosad compared with the susceptible population is 21 393, for carbosulfan 54 and for emamectin benzoate 36. Only two populations, collected from organic farms, were susceptible to the insecticides tested. CONCLUSION: This is the first report of a high resistance level to spinosad, the major insecticide used to control the onion thrips. Resistance cases to spinosad were associated with failures to control the pest. Populations resistant to spinosad also had partial or complete resistance to other insecticides used for controlling the onion thrips. Copyright © 2012 Society of Chemical Industry     

Long term and seasonal courses of leaf area index in a semi-arid forest plantation

Effective leaf area index (LAI_e) in the semi-arid Pinus halepensis plantation, located between arid and semi-arid climatic zones at the edge of the Negev and Judean deserts, was measured bi-annually during four years (2001-2004) and more intensively (monthly) during the following two years (2004-2006) by a number of non-contact optical devices. The measurements showed a gradual increase in LAI_e from ∼1 (±0.25) to ∼1.8 (±0.11) during these years. All instruments, when used properly, gave similar results that were also comparable with actual leaf area index measured by litter collection and destructive sampling and allometric estimates. Because of the constraint of clear sky conditions, which limited the use of the fisheye type sensors to times of twilight, the fisheye techniques were less useful. The tracing radiation and architecture of canopies system, which includes specific treatment of two levels of clumpiness of the sparse forest stand, was used successfully for the intensive monitoring. The mean clumpiness index, 0.61, is considered representative for the specific environment. Finally, the LAI_e measurements at the start of each season were used to constrain phenology-based estimates of annual LAI_e development, resulting in a continuous course of LAI_e in the forest over the five-year period. Intra-seasonal LAI_e variation in the order of 10% of total LAI_e predicted by the model was also observed in the intensive TRAC measurements, giving confidence in the TRAC system and indicating its sensitivity and applicability in woodlands even with low LAI_e values. The results can be important for forest management decision support as well as for use in evaluation of remote sensing techniques for forests at the lowest range of LAI_e values.     

Influence of carbonic anhydrase activity in terrestrial vegetation on the 18O content of atmospheric CO2

The oxygen-18 (18O) content of atmospheric carbon dioxide (CO2) is an important indicator of CO2 uptake on land. It has generally been assumed that during photosynthesis, oxygen in CO2 reaches isotopic equilibrium with oxygen in 18O-enriched water in leaves. We show, however, large differences in the activity of carbonic anhydrase (which catalyzes CO2 hydration and 18O exchange in leaves) among major plant groups that cause variations in the extent of 18O equilibrium (theta(eq)). A clear distinction in theta(eq) between C3 trees and shrubs, and C4 grasses makes atmospheric C18OO a potentially sensitive indicator to changes in C3 and C4 productivity. We estimate a global mean theta(eq) value of approximately 0.8, which reasonably reconciles inconsistencies between 18O budgets of atmospheric O2 (Dole effect) and CO2.     

Time and the quantum: erasing the past and impacting the future

The quantum eraser effect of Scully and Drühl dramatically underscores the difference between our classical conceptions of time and how quantum processes can unfold in time. Such eyebrow-raising features of time in quantum mechanics have been labeled "the fallacy of delayed choice and quantum eraser" on the one hand and described "as one of the most intriguing effects in quantum mechanics" on the other. In the present paper, we discuss how the availability or erasure of information generated in the past can affect how we interpret data in the present. The quantum eraser concept has been studied and extended in many different experiments and scenarios, for example, the entanglement quantum eraser, the kaon quantum eraser, and the use of quantum eraser entanglement to improve microscopic resolution.     

Effect of high temperature on quality of processing-tomatoes of various genotypes ripened on the vine

High-temperature effects on tomato fruits of various genotypes ripened on the vine in the greenhouse were studied. A significant reduction in fruit quality was found in fruits ripened in a greenhouse where the temperature was greater than 30°C for an average of 9 h/day. The parameters studied included colour, total soluble solid content and acidity. There was no significant change in the major carotenoid pigments concentrations in these fruits compared with fruits ripened at optimal temperatures. This finding was not in agreement with that obtained in an earlier study using detached fruits ripened in incubators. Diurnal temperature fluctuation and translocation processes in the plant are suggested as the major factors accounting for these differences. “White tissue”, a colour disorder, was a major factor affecting fruit quality at high temperature. Fruits of a breeding line UCX100 15-2-2 had the highest quality, did not develop colour disorders and were relatively insensitive to high-temperature effects.     

Detecting novel associations in large data sets

Identifying interesting relationships between pairs of variables in large data sets is increasingly important. Here, we present a measure of dependence for two-variable relationships: the maximal information coefficient (MIC). MIC captures a wide range of associations both functional and not, and for functional relationships provides a score that roughly equals the coefficient of determination (R(2)) of the data relative to the regression function. MIC belongs to a larger class of maximal information-based nonparametric exploration (MINE) statistics for identifying and classifying relationships. We apply MIC and MINE to data sets in global health, gene expression, major-league baseball, and the human gut microbiota and identify known and novel relationships.     

Urban environment of New York City promotes growth in northern red oak seedlings

Urbanization is accelerating across the globe, elevating the importance of studying urban ecology. Urban environments exhibit several factors affecting plant growth and function, including high temperatures (particularly at night), CO(2) concentrations and atmospheric nitrogen deposition. We investigated the effects of urban environments on growth in Quercus rubra L. seedlings. We grew seedlings from acorns for one season at four sites along an urban-rural transect from Central Park in New York City to the Catskill Mountains in upstate New York (difference in average maximum temperatures of 2.4 °C; difference in minimum temperatures of 4.6 °C). In addition, we grew Q. rubra seedlings in growth cabinets (GCs) mimicking the seasonal differential between the city and rural sites (based on a 5-year average). In the field experiment, we found an eightfold increase in biomass in urban-grown seedlings relative to those grown at rural sites. This difference was primarily related to changes in growth allocation. Urban-grown seedlings and seedlings grown at urban temperatures in the GCs exhibited a lower root: shoot ratio (urban ~0.8, rural/remote ~1.5), reducing below-ground carbon costs associated with construction and maintenance. These urban seedlings instead allocated more growth to leaves than did rural-grown seedlings, resulting in 10-fold greater photosynthetic area but no difference in photosynthetic capacity of foliage per unit area. Seedlings grown at urban temperatures in both the field and GC experiments had higher leaf nitrogen concentrations per unit area than those grown at cooler temperatures (increases of 23% in field, 32% in GC). Lastly, we measured threefold greater (13)C enrichment of respired CO(2) (relative to substrate) in urban-grown leaves than at other sites, which may suggest greater allocation of respiratory function to growth over maintenance. It also shows that lack of differences in total R flux in response to environmental conditions may mask dramatic shifts in respiratory functioning. Overall, our findings indicating greater seedling growth and establishment at a critical regeneration phase of forest development may have important implications for the ecology of urban forests as well as the predicted growth of the terrestrial biosphere in temperate regions in response to climate change.     

Effect of high temperature on quality of processing-tomatoes of various genotypes ripened off the vine

High-temperature effects on the quality of several genotypes of processing-tomatoes were studied. The quality parameters included colour, carotenoid concentration, acidity, pH and content of total soluble solids. Fruits exposed to either 27.5 or 30°C during ripening showed a significant decrease in carotenoid concentration and colour score as compared with fruits kept at 25°C. Soluble solid content did not change and acidity increased with high temperatures. Among the individual carotenoids, although lycopene concentration decreased, a more pronounced decrease occurred in the concentration of its precursors, phytoene and phytofluene. Different tomato lines responded differently to high-temperature exposure. UCX100 15-2-2 was found to be generally insensitive. However, a crimson, high pigment (og, hp) line, which is characterized by high levels of carotenoids, was found to be sensitive. Under high temperature, UCX100 15-2-2 exhibited higher fruit quality than either the og, hp line or the cultivar UC82-C in all parameters examined.     

Scanning electron microscopy of the interactions betweenerwinia herbicola pv.gypsophilae andgypsophila paniculata

Ultrastructural studies of interactions betweenErwinia herbicola pv.gypsophilae and gypsophila plants showed that the bacterial pathogen multiplied rapidly and colonized gypsophila cuttings within 2 days. Fourteen days after inoculation, the plant tissue turned tumorigenic and a gall formed. Scanning electron microscopy showedE. gypsophilae cells densely occupying the plant xylem elements. Bacteria were found in association with fibrils which might be used by bacterial cells for attachment to plant cells. Substantial numbers of fibrils were also clearly seen on calli cell surfaces.